Catalogue Number |
CYT-394 |
Synonyms |
Catabolin, Lymphocyte-activating factor (LAF), Endogenous Pyrogen (EP), Leukocyte Endogenous Mediator (LEM), Mononuclear Cell Factor (MCF), IL1F2, IL-1 beta. |
Introduction |
Interleukin-1b is produced by activated macrophages, IL-1B stimulates thymocyte proliferation by inducing il-2 release, b-cell maturation and proliferation, and fibroblast growth factor activity. IL1B proteins are involved in the inflammatory response, being identified as endogenous pyrogens, and are reported to stimulate the release of prostaglandin and collagenase from synovial cells. |
Description |
Interleukin-1b Rat Recombinant produced in E.Coli is a non-glycosylated, Polypeptide chain containing 152 amino acids and having a molecular mass of 17.3 kDa.
The IL-1b is purified by proprietary chromatographic techniques. |
Source |
Escherichia Coli. |
Physical Appearance |
Sterile Filtered White lyophilized (freeze-dried) powder. |
Formulation |
The protein was lyophilized without any additives. |
Solubility |
It is recommended to reconstitute the lyophilized Interleukin 1b in sterile 18MΩ-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions. |
Stability |
Lyophilized Interleukin-1b although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution IL1b should be stored at 4°C between 2-7 days and for future use below -18°C.
For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Please prevent freeze-thaw cycles. |
Purity |
Greater than 95.0% as determined by SDS-PAGE. |
Amino acid sequence |
VPIRQLHCRLRDEQQKCLVLSDPCELKALHLNGQNISQQVVFSMSFVQGETSND
KIPVALGLKGLNLYLSCVMKDGTPTLQLESVDPKQYPKKKMEKRFVFNKIEVKT
KVEFESAQFPNWYISTSQAEHRPVFLGNSNGRDIVDFTMEPVSS. |
Biological Activity |
The ED50 range= 0.2-0.5 ng/ml corresponding to a specific activity of 2-5MIU/mg determined by the dose dependent proliferation of mouse D10S cells. |
Protein content |
Protein quantitation was carried out by two independent methods
1. UV spectroscopy at 280 nm using the absorbency value of 0.558 as the extinction coefficient for a 0.1% (1mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics).
2. Analysis by RP-HPLC, using a standard solution of IL-1b as a Reference Standard. |
Usage |
ProSpec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals. |
Related Products |
» IL 1 Beta Antibody |
» IL 1 Beta Antibody, Biotin |
» IL 1 Beta Antibody, FITC |
» IL 1 beta Human |
» IL 1 beta Human, His |
» IL 1 beta Mon key |
» IL 1 beta Mouse |
» IL 1 beta Mouse, His |
» IL 1 beta Porcine |
|
Catalogue Number |
CYT-394 |
Synonyms |
Catabolin, Lymphocyte-activating factor (LAF), Endogenous Pyrogen (EP), Leukocyte Endogenous Mediator (LEM), Mononuclear Cell Factor (MCF), IL1F2, IL-1 beta. |
Introduction |
Interleukin-1b is produced by activated macrophages, IL-1B stimulates thymocyte proliferation by inducing il-2 release, b-cell maturation and proliferation, and fibroblast growth factor activity. IL1B proteins are involved in the inflammatory response, being identified as endogenous pyrogens, and are reported to stimulate the release of prostaglandin and collagenase from synovial cells. |
Description |
Interleukin-1b Rat Recombinant produced in E.Coli is a non-glycosylated, Polypeptide chain containing 152 amino acids and having a molecular mass of 17.3 kDa.
The IL-1b is purified by proprietary chromatographic techniques. |
Source |
Escherichia Coli. |
Physical Appearance |
Sterile Filtered White lyophilized (freeze-dried) powder. |
Formulation |
The protein was lyophilized without any additives. |
Solubility |
It is recommended to reconstitute the lyophilized Interleukin 1b in sterile 18MΩ-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions. |
Stability |
Lyophilized Interleukin-1b although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution IL1b should be stored at 4°C between 2-7 days and for future use below -18°C.
For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Please prevent freeze-thaw cycles. |
Purity |
Greater than 95.0% as determined by SDS-PAGE. |
Amino acid sequence |
VPIRQLHCRLRDEQQKCLVLSDPCELKALHLNGQNISQQVVFSMSFVQGETSND
KIPVALGLKGLNLYLSCVMKDGTPTLQLESVDPKQYPKKKMEKRFVFNKIEVKT
KVEFESAQFPNWYISTSQAEHRPVFLGNSNGRDIVDFTMEPVSS. |
Biological Activity |
The ED50 range= 0.2-0.5 ng/ml corresponding to a specific activity of 2-5MIU/mg determined by the dose dependent proliferation of mouse D10S cells. |
Protein content |
Protein quantitation was carried out by two independent methods
1. UV spectroscopy at 280 nm using the absorbency value of 0.558 as the extinction coefficient for a 0.1% (1mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics).
2. Analysis by RP-HPLC, using a standard solution of IL-1b as a Reference Standard. |
Usage |
ProSpec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals. |
Related Products |
» IL 1 Beta Antibody |
» IL 1 Beta Antibody, Biotin |
» IL 1 Beta Antibody, FITC |
» IL 1 beta Human |
» IL 1 beta Human, His |
» IL 1 beta Mon key |
» IL 1 beta Mouse |
» IL 1 beta Mouse, His |
» IL 1 beta Porcine |
|
2011年底 生化試劑-大促銷
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Human HDL 高密度脂蛋白膽固醇 Specifications :MF, AF, C (10mg/ml);Source:Human blood ;Purity:98% 2mg 1200
DiI-HDL DiI 熒光標(biāo)記高密度脂蛋白 pecifications :MF, AF , C;Source:Human blood;Purity:98% ;300ug 2300
[I125] Human HDL 碘125標(biāo)記人高密度脂蛋白pecifications :MF, AF, (100-300cpm/ng); Source:Human blood; 300uCi 2800
Human LDL人低密度脂蛋白 pecifications :MF, AF, C (2-4mg/ml;Source:Human blood;Purity :98% 2mg 1200
DiI-LDL DiI標(biāo)記人低密度脂蛋白 pecifications :MF, AF, C;Source :Human blood 300ug 2300
[I125] Human LDL 碘125標(biāo)記人低密度脂蛋白 pecifications :MF, AF, (100-300cpm/ng) , C;Source :Human blood;Purity :98% 100uCi 2600
DiO-LDL DiO標(biāo)記人低密度脂蛋白 pecifications :MF, AF, C;Source :Human blood 300ug 2700
Human Ac-LDL 人乙酰化低密度脂蛋白 pecifications :MF, AF, (2mg/ml), C;Source :Human blood;Purity :98% 2mg 1200
DiI-Ac-LDL DiI標(biāo)記乙?;说兔芏戎鞍?pecifications :MF, AF, (200ug/ml), C;Source :Human blood 200ug 2800
Dio-Ac-LDL DiO標(biāo)記乙?;说兔芏戎鞍?pecifications :MF, AF, (200ug/ml), C;Source :Human blood 300ug 2300
[I125] Human Ac-LDL 碘125標(biāo)記乙?;说兔芏戎鞍?pecifications :MF, AF, (100-300cpm/ng), C;Source :Human blood;Purity :98% 100uCi 2600
Human Ox-LDL 人氧化低密度脂蛋白 pecifications :MF, AF, Tbars, (1.5-2.5mg/ml), C;Source :Human blood;Purity :98% 2mg 1200
DiI-Ox-LDL DiI標(biāo)記人氧化低密度脂蛋白 pecifications :MF, AF, (200ug/Vial), C;Source :Human blood 300ug 2300
[I125] Ox-LDL 碘125標(biāo)記人氧化低密度脂蛋白 pecifications :MF, AF, Tbars, (100-300cpm/ng), C ;Source :Human blood;Purity :98% 100uCi 2600
Human Lp[a] 人脂蛋白a pecifications :Contains 0.01% Azide ;Source :Human blood;Purity :98% 2mg 1200
Human VLDL 人極低密度脂蛋白 pecifications :MF, AF, C (1-2mg/ml) ;Source :Human blood;Purity :98% 2mg 1200
DiI-VLDL DiI標(biāo)記的人極低密度脂蛋白
[I125] Human VLDL 碘125標(biāo)記的人極低密度脂蛋白
Lipoprotein Deficient H.Plasma 去脂蛋白血清
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