| Catalogue Number |
CYT-388 |
| Synonyms |
IFN-b2, B cell differentiation factor (BCDF), BSF-2, HPGF, HSF, MGI-2, IL-6, Interleukin HP-1, B-cell hybridoma growth factor. |
| Introduction |
Interleukin-6 is a potent pro-inflammatory cytokine primarily produced by activated T cells and an assortment of other cells including endothelial cells and macrophages. IL-6 affects B and T lymphocytes and has been shown to have a role in host defense, acute phase reactions, immune responses and hematopoiesis. |
| Description |
Interleukin-6 Rat Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 187 amino acids and having a molecular mass of 21732 Dalton.
The IL6 is purified by proprietary chromatographic techniques. |
| Source |
Escherichia Coli. |
| Physical Appearance |
Sterile Filtered White lyophilized (freeze-dried) powder. |
| Formulation |
The protein was lyophilized without any additives. |
| Solubility |
It is recommended to reconstitute the lyophilized Interleukin-6 in 100mM acetic acid to 1.0 mg/mL and incubated for 30 minutes at room temperature to regain full activity, which can then be further diluted to other aqueous solutions. |
| Stability |
Lyophilized Interleukin-6 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution IL6 should be stored at 4°C between 2-7 days and for future use below -18°C.
For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Please prevent freeze-thaw cycles. |
| Purity |
Greater than 95.0% as determined by(a) Analysis by RP-HPLC.
(b) Analysis by SDS-PAGE. |
| Amino acid sequence |
The sequence of the first five N-terminal amino acids was determined and was found to be Met-Phe-Pro-Thr-Ser. |
| Biological Activity |
The ED50= 0.03-0.1 ng/ml corresponding to a specific activity of 10-34MIU/mg. The biological activity is determined by measuring the dose-dependant proliferation of IL-6 dapendent B9 cells. A concentration range of 0.1 to 10.0 ng/mL is effective for most in vitro applications. |
| Protein content |
Protein quantitation was carried out by two independent methods
1. UV spectroscopy at 280 nm using the absorbency value of 0.55 as the extinction coefficient for a 0.1% (1mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics).
2. Analysis by RP-HPLC, using a standard solution of IL-6 as a Reference Standard. |
| Usage |
ProSpec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals. |
| Related Products |
| » IL 6 Antibody |
| » IL 6 Human |
| » IL 6 Human, CHO |
| » IL 6 Human, His |
| » IL 6 Mouse |
| » IL 6 Porcine |
| » sIL 6R Human |
|
2011年底 生化試劑-大促銷
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貨號(hào)CYT-209 重組人白介素-2 Human IL-2 10µg ￥900
貨號(hào)CYT-211重組人白介素-4 IL-4 2µg ￥900
貨號(hào)CYT-213 重組人白介素-6 IL-6 5µg ￥900
貨號(hào)CYT-216 重組人胰島素樣生長因子-1 Human IGF-1 20µg ￥900
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貨號(hào)CYT-388 重組大鼠白介素-6 rIL-6 2µg ￥900
貨號(hào)CYT-394 重組大鼠白介素-1β rIL 1 beta 2µg ￥900
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貨號(hào)CYT-446A 重組人白介素-13 IL 13 2µg ￥900
貨號(hào)CYT-569 重組人活化素-A Activin-A 2µg ￥900
貨號(hào)CYT-569 重組人活化素-A Activin-A 2µg ￥900
貨號(hào)CYT-580 重組人色素上皮衍生因子 PEDF 20µg ￥1,900
貨號(hào)ENZ-308 Taq DNA聚合酶（4支一套） TaqDNA 1,000U ￥900
貨號(hào)PRO-445 U1snRNP-68 U1snRNP-68 20µg ￥1,900
Human HDL 高密度脂蛋白膽固醇 Specifications ：MF, AF, C (10mg/ml)；Source：Human blood ；Purity：98% 2mg 1200
DiI-HDL DiI 熒光標(biāo)記高密度脂蛋白 pecifications ：MF, AF , C；Source：Human blood；Purity：98% ；300ug 2300
[I125] Human HDL 碘125標(biāo)記人高密度脂蛋白pecifications ：MF, AF, (100-300cpm/ng)； Source：Human blood； 300uCi 2800
Human LDL人低密度脂蛋白 pecifications ：MF, AF, C (2-4mg/ml；Source：Human blood；Purity ：98% 2mg 1200
DiI-LDL DiI標(biāo)記人低密度脂蛋白 pecifications ：MF, AF, C；Source ：Human blood 300ug 2300
[I125] Human LDL 碘125標(biāo)記人低密度脂蛋白 pecifications ：MF, AF, (100-300cpm/ng) , C；Source ：Human blood；Purity ：98% 100uCi 2600
DiO-LDL DiO標(biāo)記人低密度脂蛋白 pecifications ：MF, AF, C；Source ：Human blood 300ug 2700
Human Ac-LDL 人乙?；兔芏戎鞍?pecifications ：MF, AF, (2mg/ml), C；Source ：Human blood；Purity ：98% 2mg 1200
DiI-Ac-LDL DiI標(biāo)記乙?；说兔芏戎鞍?pecifications ：MF, AF, (200ug/ml), C；Source ：Human blood 200ug 2800
Dio-Ac-LDL DiO標(biāo)記乙?；说兔芏戎鞍?pecifications ：MF, AF, (200ug/ml), C；Source ：Human blood 300ug 2300
[I125] Human Ac-LDL 碘125標(biāo)記乙?；说兔芏戎鞍?pecifications ：MF, AF, (100-300cpm/ng), C；Source ：Human blood；Purity ：98% 100uCi 2600
Human Ox-LDL 人氧化低密度脂蛋白 pecifications ：MF, AF, Tbars, (1.5-2.5mg/ml), C；Source ：Human blood；Purity ：98% 2mg 1200
DiI-Ox-LDL DiI標(biāo)記人氧化低密度脂蛋白 pecifications ：MF, AF, (200ug/Vial), C；Source ：Human blood 300ug 2300
[I125] Ox-LDL 碘125標(biāo)記人氧化低密度脂蛋白 pecifications ：MF, AF, Tbars, (100-300cpm/ng), C ；Source ：Human blood；Purity ：98% 100uCi 2600
Human Lp[a] 人脂蛋白a pecifications ：Contains 0.01% Azide ；Source ：Human blood；Purity ：98% 2mg 1200
Human VLDL 人極低密度脂蛋白 pecifications ：MF, AF, C (1-2mg/ml) ；Source ：Human blood；Purity ：98% 2mg 1200
DiI-VLDL DiI標(biāo)記的人極低密度脂蛋白
[I125] Human VLDL 碘125標(biāo)記的人極低密度脂蛋白
Lipoprotein Deficient H.Plasma 去脂蛋白血清
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